Recombinant beta III Tubulin Antibody

功能和特点

规格或纯度: Recombinant (RR607); Rabbit anti Human Beta III Tubulin Antibody; WB, Flow; Unconjugated
简短描述: Rabbit recombinant (RR607) to Beta III Tubulin
反应种属: Human,Mouse ,Rat
亚型: Rabbit IgG

有货

库存信息

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库存信息

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库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab091154-50μl	50μL	现货
Ab091154-100μl	100μL	现货
Ab091154-1ml	1ml	期货

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基本信息

产品名称	Recombinant beta III Tubulin Antibody
别名	beta 3 tubulin antibody; beta 4 antibody; beta-4 antibody; CDCBM antibody; CDCBM1 antibody; CFEOM3 antibody; CFEOM3A antibody; FEOM3 antibody; M(beta)3 antibody; M(beta)6 antibody; MC1R antibody; Neuron specific beta III Tubulin antibody; Neuron-specific
英文别名	beta 3 tubulin antibody; beta 4 antibody; beta-4 antibody; CDCBM antibody; CDCBM1 antibody; CFEOM3 antibody; CFEOM3A antibody; FEOM3 antibody; M(beta)3 antibody; M(beta)6 antibody; MC1R antibody; Neuron specific beta III Tubulin antibody; Neuron-specific
规格或纯度	Recombinant (RR607); Rabbit anti Human Beta III Tubulin Antibody; WB, Flow; Unconjugated
特异性	TUBB3
反应种属	Human,Mouse ,Rat
预测反应种属	Zebrafish
交叉反应	Cross-reactivity with other species has not been measured.
免疫原	A synthetic peptide corresponding to residues on the C-terminus of human beta III tubulin was used as an immunogen.
阳性对照	WB: Jurkat, HeLa, NIH/3T3, C6, HEK293, HUVEC and A549 cell lysates. Flow: HeLa cells.
偶联标记	Unconjugated

产品属性

克隆	Recombinant
克隆号	RR607
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂	0.01% Sodium azide
浓度	0.088 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application	Notes
WB	1/2000 - 1/5000
Flow	1/200 - 1/1000

质检证书(COA)

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图片

Western Blot: Recombinant beta III Tubulin Antibody (Ab091154)

Recombinant beta III Tubulin Antibody (Ab091154) - Western Blot
All lanes: Recombinant beta III Tubulin Antibody (Ab091154) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 50 kDa
Observed band size: 51 kDa
Exposure time: 6.3 s

Flow Cytometry: Recombinant beta III Tubulin Antibody (Ab091154)

Recombinant beta III Tubulin Antibody (Ab091154) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant beta III Tubulin Antibody (Ab091154) (gold). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant beta III Tubulin Antibody (Ab091154) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Red) was used as a control.