K-562 Whole Cell Lysate

Carrier Free

Azide Free

200μg, Mycoplasma free

功能和特点

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
cl155898-200μg	200μg	现货

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K-562 (1) 裂解液 (15)

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基本描述

产品名称	K-562 Whole Cell Lysate
别名	K-562全细胞裂解物；K562全细胞裂解物
英文别名	K562 Whole Cell Lysate
规格或纯度	Carrier Free, Azide Free, 200μg, Mycoplasma free
产品介绍	Product Description： The K-562 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.

AI解读

图片

Western Blot: K562 Whole Cell Lysate (cl155898)

K562 Whole Cell Lysate (cl155898) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution

Lane 1: K562 Whole Cell Lysate (cl155898)
Lane 2: HEK293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)

Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Western Blot: K562 Whole Cell Lysate (cl155898)

K-562 Whole Cell Lysate (cl155898) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution

Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)

Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Western Blot: K562 Whole Cell Lysate (cl155898)

K-562 Whole Cell Lysate (cl155898) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution

Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 60 kDa
Observed band size: 58 kDa

产品规格参数

应用	WB
物理外观	Lyophilized
储存缓冲液	Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4)
浓度	200μg, Mycoplasma free
复溶	Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存温度	-80℃储存
运输条件	超低温冰袋运输
稳定性与储存	Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.