MCF7 Whole Cell Lysate

Carrier Free

Azide Free

200μg, Mycoplasma free

功能和特点

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
cl155899-200μg	200μg	现货

查看相关系列

MCF7 (1) 裂解液 (15)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	MCF7 Whole Cell Lysate
规格或纯度	Carrier Free, Azide Free, 200μg, Mycoplasma free
产品介绍	Product Description： The MCF7 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.

AI解读

图片

Western Blot: MCF7 Whole Cell Lysate (cl155899)

MCF7 Whole Cell Lysate (cl155899)- Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution

Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)

Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Western Blot: MCF7 Whole Cell Lysate (cl155899)

MCF7 Whole Cell Lysate (cl155899) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution

Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Western Blot: MCF7 Whole Cell Lysate (cl155899)

MCF7 Whole Cell Lysate (cl155899) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution

Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 60 kDa
Observed band size: 58 kDa

产品规格参数

应用	WB
物理外观	Lyophilized
储存缓冲液	Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
浓度	200μg, Mycoplasma free
复溶	Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存温度	-80℃储存
运输条件	超低温冰袋运输