A-431 Whole Cell Lysate

Carrier Free

Azide Free

Mycoplasma free

功能和特点

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
cl155901-200μg	200μg	现货

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A-431 (1) 裂解液 (15)

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基本描述

产品名称	A-431 Whole Cell Lysate
别名	A-431全细胞裂解物; A431全细胞裂解物
英文别名	A431 Whole Cell Lysate
规格或纯度	Carrier Free, Azide Free, Mycoplasma free
产品介绍	Product Description： The A-431 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. A-431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

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图片

Western Blot: A-431 Whole Cell Lysate (cl155901)

A-431 Whole Cell Lysate (cl155901) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: C6 Whole Cell Lysate (cl155903)
Lane 2: A-431 Whole Cell Lysate (cl155901)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 42.0 kDa
Observed band size: 42.0 kDa

Western Blot: A-431 Whole Cell Lysate (cl155901)

A-431 Whole Cell Lysate (cl155901) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/1000 dilution
Lane 1: C6 Whole Cell Lysate (cl155903)
Lane 2: A-431 Whole Cell Lysate (cl155901)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 29.0 kDa
Observed band size: 29.0 kDa

Western Blot: A-431 Whole Cell Lysate (cl155901)

A-431 Whole Cell Lysate (cl155901) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: C6 Whole Cell Lysate (cl155903)
Lane 2: A-431 Whole Cell Lysate (cl155901)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 60.0 kDa
Observed band size: 58.0 kDa

产品规格参数

应用	WB
物理外观	Lyophilized
储存缓冲液	Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
浓度	Mycoplasma free
复溶	Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存温度	-80℃储存
运输条件	超低温冰袋运输
稳定性与储存	Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.