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基本描述
| 规格或纯度 | 0.1-10 kb, 21 bands |
|---|---|
| 稳定性与储存 | 4℃保存，6个月有效。-20℃保存，至少一年有效。 |
| 英文名称 | DNA Ladder |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
产品介绍： DNA Ladder (0.1-10 kb, 21 bands)是一种即用型(ready for use)DNA分子量标准(DNA Molecular Weight Marker)，包含了1 Kb DNA Ladder和100bp DNA ladder共21条双链DNA条带，可以满足各种常规DNA电泳分析的分子量参照需要。本产品类似于Invitrogen等公司的1 Kb plus DNA ladder，但比Invitrogen公司的1 Kb plus DNA ladder的条带分布更加均匀细致，便于对照DNA分子量。本产品中500bp、1000bp和3000bp条带用黑体标记(参见右图)，亮度较高，使辨别条带更加容易。具体每条带的DNA的含量可以参考右图进行计算。本DNA ladder中已添加了含有溴酚蓝的DNA上样缓冲液，可以直接使用。每孔上样5微升，即可观察到非常清楚的DNA条带(参见右图)。注意事项： 如果使用强致癌的溴化乙锭(EB)，须注意避免DNA ladder在使用时被溴化乙锭污染，操作时一定要采取适当的防护措施。推荐使用无毒的EB升级换代产品NA-Red 、NA-Green 、Gel-Red 和Gel-Green 。电泳时请尽量使用未用过的电泳缓冲液和新配制的琼脂糖凝胶，以免影响电泳效果。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。使用说明： 1.DNA琼脂糖凝胶电泳时直接加入5微升DNA ladder至加样孔中，即可电泳。2.如发现溴酚蓝对DNA条带的观察有干扰，请换用D0109 DNA Ladder (0.1-10 kb, 21 bands, with Red)。DNA Ladder (0.1-10 kb, 21 bands) is supplied in a ready-to-use format containing bromophenol blue and 21 double-stranded DNA fragments ranging from 100bp to 10kb. It can meet the molecular weight reference needs for most regular DNA electrophoresis analysis.The intensity of 500bp, 1000bp, and 3000bp fragments is higher than others, making the ladder bands easier to be differentiated. The content of each ladder fragment is shown on the right side of the Figure, allowing the estimation of both size and concentration of DNA fragments on agarose gels.Loading 5μl of DNA Ladder per lane is sufficient to visualize all fragments of the ladder. Precautions： If ethidium bromide (EB), a strong carcinogen, is used for DNA electrophoresis, please take care to avoid the contamination of this product by EB during frequent uses. We recommend using non-toxic, upgraded EB products such as NA-Red (, D0128, D0130), NA-Green , Gel-Red and Gel-Green .Please use new electrophoresis buffer and freshly prepared agarose gel for electrophoresis to ensure better DNA resolution .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use： 1.For DNA agarose gel electrophoresis, add 5μl of this product directly into an empty well on agarose gel.2.If bromophenol blue interferes with the observation of target DNA, the DNA Ladder (0.1-10 kb, 21 bands, Red) (, D0109) can be used.Related Products:Cat. No.Product NamePack SizeD0107DNA Ladder (0.1-10 kb, 21 bands)100TD0109DNA Ladder (0.1-10 kb, 21 bands, with Red)100TD0110DNA Ladder (0.2-12 kb, 12 bands)100TD0111DNA Ladder (0.2-12kb, 12 bands, with Red)100T |
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