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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| E743381-100ml |
100ml |
期货  |
| |
| E743381-500ml |
500ml |
期货 |
|
| 稳定性与储存 | 4℃保存，一年有效。-20℃可以长期保存，室温保存一个月内有效。 |
|---|---|
| 英文名称 | Enhanced Immunostaining Permeabilization Buffer |
| 储存温度 | 2-8°C储存 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
产品介绍： 免疫染色强力通透液(Enhanced Immunostaining Permeabilization Buffer, 也称Enhanced ImmunostainingPermeabilization Solution)，可以用于免疫染色等多种原位检测时细胞样品、冰冻或石蜡切片的通透处理，有助于暴露抗原、核酸等作用靶点，使抗体、探针或标记物等更容易进入细胞内，从而确保并增强染色等的检测效果。本产品为即用型工作液，可以直接使用，无需稀释。本产品通透能力强，推荐用于各种常规的免疫荧光、免疫组化、免疫细胞化学、流式检测、细胞凋亡的TUNEL检测、阿拉丁的UltraBio™检测等中细胞的通透，特别适合需要强力通透细胞膜的情况。对于通透效果要求不高的情况，也可以使用阿拉丁的免疫染色洗涤液或免疫染色通透液(Triton X-100) 。对于需要检测细胞膜蛋白的情况推荐使用免疫染色通透液(Saponin) 。对于凝集素(lectin)的检测推荐使用免疫染色通透液(Triton X-100) 或本产品。通透液通常可以通过使用有机溶剂例如甲醇、丙酮等，或者使用去垢剂例如Triton X-100、Saponin等来实现对于细胞膜的通透。甲醇或丙酮等有机溶剂，一方面可以溶解细胞膜和核膜从而充分暴露细胞浆和细胞核中的目的蛋白等，另一方面也可以使细胞内的蛋白变性起到固定的作用。甲醇或丙酮等有机试剂操作比较简单，一步处理可以同时实现固定和通透，缺点是膜蛋白也会被溶解掉并且有些蛋白变性后不利于后续的检测，因此有机溶剂使用相对较少，仅用于一些要求比较粗糙的检测。Triton X-100是常用的通透试剂，可以通透细胞膜和核膜，作用的原理也是非特异性地溶解细胞膜，因此其缺点是不利于膜蛋白的检测，但当使用多聚甲醛进行交联和固定后，相当一部分膜蛋白会被交联固定，从而不会被Triton X-100溶解，因此后续仍然能检测到。Saponin可以特异性地溶解细胞膜中的胆固醇，从而实现在细胞膜上选择性地打孔，其优点是适合于细胞膜蛋白的检测，特别适合通过流式细胞仪检测细胞膜上的标志性蛋白，缺点是对于一些胆固醇含量低的细胞通透效果差，通透效果弱于Triton X-100和有机溶剂，并且不能通透胆固醇含量很低的核膜和线粒体膜。对于凝集素(lectin)的检测，含Triton X-100等非特异性去垢剂的通透液的效果显著优于主要含Saponin的通透液。本免疫染色强力通透液中含有Triton X-100等去垢剂，配制在PBS中。经本免疫染色强力通透液处理的样品，对于细胞浆或细胞核内目的蛋白的免疫染色检测结果表明，染色效果与常规的通透液相比基本一致或有显著增强。按照每个样品需要0.1或1毫升免疫染色强力通透液计算，一个100ml包装的本产品可以通透1000个或100个样品，一个500ml包装的本产品可以通透5000个或500个样品。注意事项： 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。使用说明： 1.对于切片，在固定、洗涤结束后，每样滴50-100μl免疫染色强力通透液，也可以在染色缸中完全浸没进行通透。对于细胞样品，在固定、洗涤结束后，按照六孔板每孔加入1毫升免疫染色强力通透液，其它多孔板参考该比例加入。对于其它样品，加入免疫染色强力通透液的量以充分盖住样品为准。2.通常在免疫染色强力通透液中室温孵育5-10分钟，即可完成通透。对于较难通透的样品或者要求通透特别充分的情况，可以在免疫染色强力通透液中室温孵育10-30分钟。Aladdin's Enhanced Immunostaining Permeabilization Buffer can be used for permeabilization of cell samples, frozen or paraffin sections in in situ assays such as immunostaining to expose the detection targets such as antigens and nucleic acids, allowing for efficient target labeling by antibodies, probes, or other markers. This ready-to-use product can be used directly without any extra preparations.This product has strong permeability and is recommended for cell permeation in various routine immunofluorescence, immunohistochemistry, immunocytochemistry, flow cytometry assays, TUNEL assays, and UltraBio™ assays, etc. It is especially suitable for samples where strong cell membrane permeation is required. For low permeation requirements, Aladdin's Immunostaining Wash Buffer or Immunostaining Permeabilization Buffer with Triton X-100 can also be used. For detection of cell membrane proteins, immunostaining permeabilization buffer with Saponin is recommended. For lectin detection, we recommend using this product or Immunostaining Permeabilization Buffer with Triton X-100 .Permeabilization of cell membranes is usually achieved by using organic solvents such as methanol and acetone, or by using detergents such as Triton X-100 and Saponin. Organic solvents can lyse cell membrane and nuclear membrane to fully expose target proteins in cell plasma and nucleus, and also denature proteins in the meantime as a fixative. The disadvantage is that membrane proteins are also dissolved and some proteins are denatured, which is unfavorable sometimes for downstream applications. Therefore, organic solvents are rarely used except for some rough assays. Triton X-100 is a commonly used permeabilizing reagent that can permeate cell and nuclear membranes by non-specific lysis of cell membranes, thus its disadvantage is that it is not conducive for detection of membrane proteins. However, after cross-linking and fixation with paraformaldehyde, a significant portion of membrane proteins will not be lysed by Triton X-100 and can be detected subsequently. Saponin specifically solubilizes cholesterol in cell membranes and thus enables selective perforation on cell membranes. Its advantage is that it is suitable for examining cell membrane proteins, especially signature proteins on cell membranes by flow cytometry. Its disadvantage is that it has weaker permeability than Triton X-100 and organic solvents in permeabilizing cells with low cholesterol content, and cannot penetrate nuclear and mitochondrial membranes with very low cholesterol content. For the detection of lectin, permeabilization solutions containing non-specific detergents such as Triton X-100 are significantly more effective than those containing Saponin mainly.This product contains Triton X-100 and other detergents and is prepared in PBS. Immunostaining assay result of target cytosol or nuclear proteins in samples treated with this product is basically the same or even superior to that obtained with conventional permeabilization solutions. Precautions： This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use： 1. For slice sections, after fixation and washing, add 50-100μl of this product per sample, or permeabilize by complete submersion in this product in a staining jar. For cell samples, after fixation and washing, add 1ml of this product per well for six-well plates, or refer to this ratio for cells in other multi-well plates. For other samples, add sufficient Immunostaining Permeabilization Buffer to cover samples completely.2. Permeabilization can usually be completed by incubation at room temperature for 5-10 minutes. For samples that are more difficult to permeate or require full permeation particularly, incubate for 10-30 minutes at room temperature. |
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