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基本描述
| 稳定性与储存 | 室温避光保存，至少一年有效。 |
|---|---|
| 英文名称 | Nissl Staining Solution |
| 储存温度 | 避光,室温 |
| 运输条件 | 常规运输 |
| 产品介绍 |
产品介绍： 阿拉丁生产的尼氏(Nissl)染色液(Nissl Staining Solution)是神经生物学家广泛使用的一种Nissl染色液，用于石蜡或冰冻切片神经元细胞浆中的尼氏小体(Nissl body)染色。Nissl染色是以德国的精神病学家和神经病理学家Franz Nissl的名字命名的。Nissl染色液染色后呈蓝紫色，常用于显示脑或脊髓的基本神经结构。Nissl小体大而数量多，说明神经细胞合成蛋白质的功能较强；相反在神经细胞受到损伤时，Nissl小体的数量会减少甚至消失。本Nissl染色液染色的有效成分是Cresyl violet。Cresyl violet可以和RNA或DNA结合，可以染色粗面型内质网上的核糖体，也可以染色细胞核。染色后使细胞体呈现斑驳的(mottled)蓝紫色染色。一个包装的本染色液至少可以染色200个样品。注意事项： 特别注意：Nissl染色液的染色能力很强，并且染色后很难去除，请注意勿使染色液沾染皮肤和衣物等。需自备4%多聚甲醛、70%乙醇和95%乙醇。如果需要脱水、透明和封片处理，还需自备二甲苯，中性树胶或其它封片剂。如果样品是石蜡切片，需自备90%乙醇，无水乙醇以及二甲苯。样品数量较多时，可以使用阿拉丁生产的染色架和染色缸，便于操作。第一次使用本试剂盒时建议先取1-2个样品做预实验。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。使用说明： 1.样品处理a.对于石蜡切片：二甲苯中脱蜡5-10分钟，共三次。注：脱蜡不充分会导致染色不均匀。无水乙醇5分钟。90%乙醇2分钟。70%乙醇2分钟。蒸馏水2分钟。b.对于冰冻切片：用4%多聚甲醛固定10分钟以上。蒸馏水洗涤2分钟。换用新鲜的蒸馏水，再洗涤2分钟。c.对于培养细胞：用4%多聚甲醛固定10分钟以上。蒸馏水洗涤2分钟。换用新鲜的蒸馏水，再洗涤2分钟。2.尼氏(Nissl)染色对于上述处理好的样品：尼氏(Nissl)染色液染色染色3-10分钟(可以根据染色结果和要求调整时间，染色时温度提高到37-50℃对于25-50微米等较厚的切片可以使染色更均匀）。蒸馏水洗涤2次(每次数秒钟即可)。95%乙醇约5秒。此时，如果需要直接观察，可以用70%乙醇洗涤2次。如需脱水、透明后封片按后续步骤进行，70%乙醇洗涤后仍可按照后续步骤进行脱水、透明和封片处理。注：如果用于免疫组化等染色后的复染，可以参考上述步骤在其它染色完成后直接进行尼氏(Nissl)染色。3.脱水、透明、封片或进行其它染色a.脱水、透明、封片：95%乙醇脱水2分钟。换用新鲜的95%乙醇再脱水2分钟。二甲苯透明5分钟。换用新鲜的二甲苯，再透明5分钟。用中性树胶或其它封片剂封片。显微镜下观察，细胞呈现斑驳的蓝紫色染色。b. 进行其它染色：如果进行免疫荧光染色，或进行Hoechst等荧光染料的染色，在Nissl染色液染色后：70%乙醇洗涤2次，每次2分钟。PBS或生理盐水或TBS或TBST等用于免疫染色或荧光染料染色的溶液浸泡5分钟。然后就可以进行免疫荧光染色或其它荧光染料的染色了。Aladdin's Nissl Staining Solution can be used to stain Nissl bodies in plasma of neuron cells preserved in paraffin or frozen sections. The Nissl Staining Solution contains Cresyl violet which can bind to RNA on the rough endoplasmic reticulum and DNA in nucleus and stain cell body in mottled blue-purple.Nissl Stain named after Franz Nissl, a German psychiatrist and neuropathologist, stains Nissl bodies in blue-purple, and is often used by neurobiologist to display the basic neural structure of brain or spinal cord. A large number of Nissl bodies indicates that nerve cells are active in protein synthesis, while the number of Nissl bodies will decrease or even disappear in damaged nerve cells.This product is sufficient for staining 200 samples. Precautions： Special Note: Nissl stain has a strong staining capability and is difficult to remove. Please take effective measures to avoid contact with skin or clothing.When there is a large number of samples, staining racks or tanks produced by can be used for easier operation.For first-time users of this product, we recommend performing a preliminary test with 1-2 samples.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear lab coat and disposable gloves during the operation.Instructions for Use： 1. Sample preparationa. For paraffin sections:Dewax paraffin sections thrice in xylene for 5-10 minutes each. Note: Insufficient dewaxing will cause uneven sample staining.Anhydrous ethanol for 5 minutes;90% ethanol for 2 minutes;70% ethanol for 2 minutes;Distilled water for 2 minutes;Proceed to step 2.b. For frozen sections:Fix frozen sections with 4% paraformaldehyde for more than 10 minutes;Wash sections with distilled water for 2 minutes;Replace with new distilled water and wash for another 2 minutes;Proceed to step 2.c. For cultured cells:Fix cells with 4% paraformaldehyde for more than 10 minutes;Wash with distilled water for 2 minutes;Replace with fresh distilled water and wash for another 2 minutes;Proceed to step 2.2. Nissl staininga. Stain samples with Nissl Staining Solution for 5-10 minutes (The duration of time can be adjusted appropriately).b. Rinse with distilled water twice for a few seconds each time.c. Wash with 95% ethanol for 5 seconds.d. Wash samples with 70% ethanol twice, examine cells directly or proceed to step 3a or step 3b.Note: To counterstain sample that has been immunohistochemistry stained, the above procedures can be performed directly after the completion of immunohistochemistry staining.3. Dehydration, clearing, mounting or fluorescence staininga. Dehydration, clearing, and mountingDehydrate samples with 95% ethanol for 2 minutes;Replace with fresh 95% ethanol and dehydrate for another 2 minutes;Replace with xylene and clear for 5 minutes;Replace with fresh xylene and clear for another 5 minutes;Use neutral gum or other mounting medium to mount the slide;Examine samples by light microscopy. Cells should be stained in mottled blue-purple.b. Fluorescence stainingAfter staining with Nissl, immunofluorescence staining or other fluorescence staining, such as Hoechst staing, can be performed as follows:Following step 2d, wash samples with 70% ethanol twice, 2 minutes each.Immerse samples for 5 minutes in PBS, saline, TBS or TBST solution used for immunostaining or fluorescence staining.Proceed to immunofluorescence staining or other fluorescence staining following appropriate protocols. |
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