Recombinant Human Siglec-2/CD22 Protein

Animal Free11

Carrier Free

生物活性级

ActiBioPure™

≥90%(SDS-PAGE)

功能和特点

表达系统: HEK293
Accession #: P20273
蛋白标签: C-His
生物活性: Immobilized Recombinant Human Siglec-2/CD22 Protein (rp169575) at 1.0 μg/mL can bind Inotuzumab (anti-CD22) (Ab176429) with the EC50 of 20.1 ng/mL. Immobilized Recombinant Human Siglec-2/CD22 Protein (rp169575) at 1.0 μg/mL can bind bind Epratuzumab (ant
内毒素水平: <1 EU/μg

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp169575-10μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	10μg	现货
rp169575-50μg	50μg	现货
rp169575-100μg	100μg	期货
rp169575-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CD22

Accession#:P20273 (10) CD22 (11) Gene ID:933 (10) Siglec-2/CD22 (1)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	Recombinant Human Siglec-2/CD22 Protein
别名	重组人Siglec-2/CD22蛋白 \| 重组人Siglec-2/CD22蛋白
英文别名	B-cell receptor CD22 \| BL-CAM \| B-lymphocyte cell adhesion molecule \| CD22 antigenMGC130020 \| CD22 molecule \| CD22 \| sialic acid binding Ig-like lectin 2 \| Sialic acid-binding Ig-like lectin 2 \| Siglec2 \| Siglec-2 \| SIGLEC2FLJ22814 \| T-cell surface antige
规格或纯度	Animal Free, Carrier Free, Bioactive, ActiBioPure™, ≥90%(SDS-PAGE)
产品介绍	Purity: ≥90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the identified Siglecs 5 to 11. To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. The predominant Siglec-2/CD22 beta encodes an 847 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail with 4 immunoreceptor tyrosine-based inhibition motifs (ITIMs). The variant Siglec-2/CD22 alpha encodes a 647 aa polypeptide missing two Ig-like C2-type domains and has a truncated (23 aa) cytoplasmic tail. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis. Besides its role as an adhesion molecule, Siglec-2/CD22 is a coreceptor that physically interacts with B-cell receptor (BCR) and is rapidly phosphorylated upon BCR ligation. It negatively regulates BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2/CD22 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling.
生物活性	Immobilized Recombinant Human Siglec-2/CD22 Protein (rp169575) at 1.0 μg/mL can bind Inotuzumab (anti-CD22) (Ab176429) with the EC50 of 20.1 ng/mL. Immobilized Recombinant Human Siglec-2/CD22 Protein (rp169575) at 1.0 μg/mL can bind bind Epratuzumab (ant
内毒素水平	<1 EU/μg
表达系统	HEK293
氨基酸	1-687 aa
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P20273
来源	Recombinant
预测分子量	75.8 kDa
SDS-PAGE	115.2 kDa, under reducing conditions; 106.3 kDa, under non-reducing conditions

图片

ELISA: Recombinant Human Siglec-2/CD22 Protein (rp169575)

Recombinant Human Siglec-2/CD22 Protein (rp169575) - ELISA
Immobilized Recombinant Human Siglec-2/CD22 Protein (rp169575) at 1.0 μg/mL can bind Inotuzumab (anti-CD22) (Ab176429) with the EC50 of 20.1 ng/mL.

ELISA: Recombinant Human Siglec-2/CD22 Protein (rp169575)

Recombinant Human Siglec-2/CD22 Protein (rp169575) - ELISA
Immobilized Recombinant Human Siglec-2/CD22 Protein (rp169575) at 1.0 μg/mL can bind bind Epratuzumab (anti-CD22) (Ab175532) with the EC50 of 207.0 ng/mL.

SDS-PAGE: Recombinant Human Siglec-2/CD22 Protein (rp169575)

Recombinant Human Siglec-2/CD22 Protein (rp169575) - SDS-PAGE
3 μg/lane of Recombinant Human Siglec-2/CD22 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 115.2 kDa under reducing conditions and 106.3 kDa under non-reducing conditions.

产品规格参数

储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C for 1 year. Avoid freeze / thaw cycle.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	日期	货号
ZJ24F0606791	分析证书	24-06-14	rp169575
ZJ24F0606790	分析证书	24-06-14	rp169575
ZJ24R0500608	分析证书	24-06-02	rp169575

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Human Siglec-2/CD22 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

AI解读

图片

产品规格参数

技术规格说明书

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关文档

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器