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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab088306-20μl |
20μl |
期货 ![]() |
| |
Ab088306-50μl |
50μL |
现货 ![]() |
| |
Ab088306-100μl |
100μL |
现货 ![]() |
| |
Ab088306-1ml |
1ml |
期货 ![]() |
|
产品名称 | ATP1A1 Mouse mAb |
---|---|
别名 | alpha 1 Sodium Potassium ATPase antibody; A1A1 antibody; AT1A1 antibody; AT1A1_HUMAN antibody; ATP1A1 antibody; Atpa-1 antibody; ATPase Na+/K+ transporting alpha 1 polypeptide antibody; ATPase Na+/K+ transporting subunit alpha 1 antibody; BC010319 antibod |
英文别名 | alpha 1 Sodium Potassium ATPase antibody; A1A1 antibody; AT1A1 antibody; AT1A1_HUMAN antibody; ATP1A1 antibody; Atpa-1 antibody; ATPase Na+/K+ transporting alpha 1 polypeptide antibody; ATPase Na+/K+ transporting subunit alpha 1 antibody; BC010319 antibod |
规格或纯度 | mAb (C209); Mouse anti Human ATP1A1 Antibody; WB, IHC; Unconjugated |
特异性 | ATP1A1 |
反应种属 | Bovine,Canine,Caprine,Equine,Human,Mouse ,Ovine,Porcine,Rabbit ,Rat |
免疫原 | Recombinant ATP1a1 (His493~Ala660) expressed in E.coli |
阳性对照 | WB: HEK293, Hep G2 and C6 cell lysates. IHC: Human Placenta Tissue, Human Liver cancer Tissue, Human Kidney Tissue, Porcine Kidney Tissue, Rat Uterus Tissue, Rat Small intestine Tissue. |
偶联标记 | Unconjugated |
克隆 | Monoclonal |
---|---|
克隆号 | C209 |
Format | Whole IgG |
亚型 | IgG2b |
轻链亚型 | Kappa |
SDS-PAGE | 150kDa |
纯化方法 | Protein A + Protein G affinity chromatography |
物理外观 | Liquid |
储存缓冲液 | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. |
防腐剂 | 0.02% NaN3 |
浓度 | 1mg/mL |
储存温度 | -20°C储存,避免反复冻融 |
运输条件 | 超低温冰袋运输 |
稳定性与储存 | Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Application | Notes |
---|---|
Western blotting |
0.5-2µg/mL
|
Immunohistochemistry |
5-20µg/mL
|
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ATP1A1 Mouse mAb (Ab088306) - Western Blot
All lanes: ATP1A1 Mouse mAb (Ab088306) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 95 kDa
Exposure time: 117.2 s
ATP1A1 Mouse mAb (Ab088306) - Western Blot
All lanes: ATP1A1 Mouse mAb (Ab088306) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 100 kDa
Observed band size: 95 kDa
Exposure time: 30.0 s
ATP1A1 Mouse mAb (Ab088306) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC
DAB staining on IHC
Sample: Human Liver cancer Tissue
Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC
DAB staining on IHC
Sample: Porcine Kidney Tissue
Primary Ab: 20 µg/mL ATP1A1 Mouse mAb (Ab088306)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC
DAB staining on IHC
Sample: Rat Uterus Tissue
Primary Ab: 3 µg/mL ATP1A1 Mouse mAb (Ab088306)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC
DAB staining on IHC
Sample: Rat Small intestine Tissue
Primary Ab: 3 ug/mL ATP1A1 Mouse mAb (Ab088306)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.