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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab088443-20μl |
20μl |
期货 ![]() |
| |
Ab088443-50μl |
50μL |
现货 ![]() |
| |
Ab088443-100μl |
100μL |
现货 ![]() |
| |
Ab088443-1ml |
1ml |
期货 ![]() |
|
产品名称 | alpha-Smooth Muscle Actin Mouse mAb |
---|---|
别名 | A actin antibody; AAT6 antibody; ACTA_HUMAN antibody; ACTA2 antibody; Actin alpha 2 smooth muscle aorta antibody; Actin aortic smooth muscle antibody; Actin, aortic smooth muscle antibody; ACTSA antibody; ACTVS antibody; Alpha 2 actin antibody; Alpha acti |
英文别名 | A actin antibody; AAT6 antibody; ACTA_HUMAN antibody; ACTA2 antibody; Actin alpha 2 smooth muscle aorta antibody; Actin aortic smooth muscle antibody; Actin, aortic smooth muscle antibody; ACTSA antibody; ACTVS antibody; Alpha 2 actin antibody; Alpha acti |
规格或纯度 | mAb (F1); Mouse anti Human alpha-Smooth Muscle Actin Antibody; WB, IHC; Unconjugated |
特异性 | Alpha smooth muscle Actin |
反应种属 | Human,Mouse ,Rat |
免疫原 | Recombinant ACTA2 expressed in E.coli (AA 167-377). |
阳性对照 | WB: NIH/3T3, Mouse skeletal and Mouse heart lysates; IHC: Human Ovarian cancer tissues, Human Rectum tissue, Rat Skeletal muscle tissues, Rat Lung tissue, Mouse Pancreas tissues, Mouse Spleen tissues. |
偶联标记 | Unconjugated |
克隆 | Monoclonal |
---|---|
克隆号 | F1 |
Format | Whole IgG |
亚型 | Mouse IgG1 |
轻链亚型 | Kappa |
SDS-PAGE | 150 kDa |
纯化方法 | Protein A + Protein G purified |
物理外观 | Liquid |
储存缓冲液 | 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4 |
防腐剂 | 0.02% Sodium azide |
浓度 | Lot by Lot |
储存温度 | -20°C储存,避免反复冻融 |
运输条件 | 超低温冰袋运输 |
稳定性与储存 | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Application | Notes |
---|---|
IHC | 5-20 µg/mL |
WB |
0.001-2 μg/mL
|
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Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot
All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5.6 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot
All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 26.7 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Human Ovarian cancer Tissue
Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse
IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Human Rectum Tissue
Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Rat Skeletal muscle Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Rat Lung Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Mouse Pancreas Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Mouse Spleen Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody