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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab094381-20μl |
20μl | 期货 | | |
Ab094381-50μl |
50μL | 现货 | | |
Ab094381-100μl |
100μL | 现货 | | |
Ab094381-1ml |
1ml | 期货 | |
产品名称 | CD276 Antibody |
---|---|
别名 | 4Ig B7 H3 antibody; 4Ig-B7-H3 antibody; AU016588 antibody; B7 H3 antibody; B7 homolog 3 antibody; B7-H3 antibody; B7H3 antibody; B7RP-2 antibody; CD_antigen=CD276 antibody; CD276 antibody; CD276 antigen antibody; CD276 molecule antibody; CD276_HUMAN antib |
英文别名 | 4Ig B7 H3 antibody; 4Ig-B7-H3 antibody; AU016588 antibody; B7 H3 antibody; B7 homolog 3 antibody; B7-H3 antibody; B7H3 antibody; B7RP-2 antibody; CD_antigen=CD276 antibody; CD276 antibody; CD276 antigen antibody; CD276 molecule antibody; CD276_HUMAN antib |
规格或纯度 | pAb; Rabbit anti Human CD276 Antibody; WB, IHC, Flow; Unconjugated |
特异性 | CD276 |
反应种属 | Human |
免疫原 | Recombinant CD276 expressed in E.coli (AA 47-219) |
阳性对照 | WB: HeLa, LNCAP, HEK293 and MCF7 cell lysates. IHC: Human Liver cancer tissue, Human Kidney tissue. Flow: CHO-K1 transfected with Human CD276. |
偶联标记 | Unconjugated |
克隆 | Polyclonal |
---|---|
Format | Whole IgG |
亚型 | Rabbit IgG |
SDS-PAGE | 150 kDa |
纯化方法 | Antigen affinity purified and Protein A purified |
物理外观 | Liquid |
储存缓冲液 | 10 mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4 |
防腐剂 | 0.05% Proclin 300 |
浓度 | Lot by Lot |
储存温度 | -20°C储存,避免反复冻融 |
运输条件 | 超低温冰袋运输 |
稳定性与储存 | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Application | Notes |
---|---|
IHC | 5-30 µg/mL |
WB | 0.01-3 µg/mL |
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CD276 Antibody (Ab094381) - Western Blot
All lanes: CD276 Antibody (Ab094381) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 92 kDa
Exposure time: 71.7 s
CD276 Antibody (Ab094381) - Flow Cytometry
CHO-K1 transfected with human CD276 (Stable transfection) was stained with CD276 Antibody (Ab094381) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by Cy5-conjugated Anti-rabbit IgG Secondary Antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
CD276 Antibody (Ab094381) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 μg/mL CD276 Antibody (Ab094381)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
CD276 Antibody (Ab094381) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 μg/mL CD276 Antibody (Ab094381)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody