Recombinant GAPDH Antibody

功能和特点

规格或纯度: Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
简短描述: Rabbit recombinant (RR688) to GAPDH
反应种属: Human,Mouse
亚型: Rabbit IgG

有货

库存信息

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库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab104788-50μl	50μL	现货
Ab104788-100μl	100μL	现货
Ab104788-1ml	1ml	期货

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基本信息

产品名称	Recombinant GAPDH Antibody
别名	38 kDa BFA-dependent ADP-ribosylation substrate antibody; aging associated gene 9 protein antibody; Aging-associated gene 9 protein antibody; BARS-38 antibody; cb609 antibody; EC 1.2.1.12 antibody; Epididymis secretory sperm binding protein Li 162eP antib
英文别名	38 kDa BFA-dependent ADP-ribosylation substrate antibody; aging associated gene 9 protein antibody; Aging-associated gene 9 protein antibody; BARS-38 antibody; cb609 antibody; EC 1.2.1.12 antibody; Epididymis secretory sperm binding protein Li 162eP antib
规格或纯度	Recombinant (RR688); Rabbit anti Human GAPDH Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated
特异性	GAPDH
反应种属	Human,Mouse
预测反应种属	Chicken,Chimpanzee,Gorilla,Guinea pig,Pig,Rat,Reptile,Rhesus monkey,Turkey,Xenopus laevis
交叉反应	Cross-reactivity with other species has not been measured.
免疫原	A synthetic peptide corresponding to residues of human GAPDH was used as an immunogen (AA 200-300).
阳性对照	WB: A431, MCF-7, A549, HEK293, HeLa, Raji, Jurkat, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Prostate cancer tissue. Flow: HeLa cells. IF/ICC: HeLa cells. IP: HeLa whole cell lysate.
偶联标记	Unconjugated

产品属性

克隆	Recombinant
克隆号	RR688
Format	Whole IgG
亚型	Rabbit IgG
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂	0.01% Sodium azide
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application	Notes
IP	1/50
IHC	1/1600-1/3200
Flow	1/200 - 1/1000
WB	1/5000 - 1/40000
IF/ICC	1/2000 - 1/10000

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质检报告(COA)

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图片

Western Blot: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - Western Blot
All lanes: Recombinant GAPDH Antibody (Ab104788) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 1.0 s

IF: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IF
IF analysis of GAPDH (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant GAPDH Antibody (Ab104788) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

IHC: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of prostate cancer tissue labelling GAPDH with Recombinant GAPDH Antibody (Ab104788) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow Cytometry: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant GAPDH Antibody (Ab104788) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant GAPDH Antibody (Ab104788) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

IP: Recombinant GAPDH Antibody (Ab104788)

Recombinant GAPDH Antibody (Ab104788) - IP
GAPDH was immunoprecipitated from 0.1 mg of HeLa whole cell lysate with Recombinant GAPDH Antibody (Ab104788) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant GAPDH Antibody (Ab104788) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant GAPDH Antibody (Ab104788) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 2 μg (input).