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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab120681-50μl |
50μL |
现货 ![]() |
| |
Ab120681-100μl |
100μL |
现货 ![]() |
| |
Ab120681-1ml |
1ml |
期货 ![]() |
|
产品名称 | Recombinant PCNA Antibody |
---|---|
别名 | ATLD2 antibody; cb16 antibody; Cyclin antibody; DNA polymerase delta auxiliary protein antibody; etID36690.10 antibody; fa28e03 antibody; fb36g03 antibody; HGCN8729 antibody; MGC8367 antibody; Mutagen-sensitive 209 protein antibody; OTTHUMP00000030189 ant |
英文别名 | ATLD2 antibody; cb16 antibody; Cyclin antibody; DNA polymerase delta auxiliary protein antibody; etID36690.10 antibody; fa28e03 antibody; fb36g03 antibody; HGCN8729 antibody; MGC8367 antibody; Mutagen-sensitive 209 protein antibody; OTTHUMP00000030189 ant |
规格或纯度 | Recombinant (RR704); Rabbit anti Human PCNA Antibody; WB, Flow, IP; Unconjugated |
特异性 | PCNA |
反应种属 | Human |
预测反应种属 | Mouse,Rat |
交叉反应 | Cross-reactivity with other species has not been measured. |
免疫原 | A synthetic peptide corresponding to residues of human PCNA was used as an immunogen (AA 100-200). |
阳性对照 | WB: A431, HEK293, MCF7, HeLa, Hep G2, NIH/3T3, C6, K562, Jurkat and HUVEC cell lysates. Flow: HeLa cells. IP: HeLa whole cell lysate. IF/ICC: HeLa cells. |
偶联标记 | Unconjugated |
克隆 | Recombinant |
---|---|
克隆号 | RR704 |
Format | Whole IgG |
亚型 | Rabbit IgG |
SDS-PAGE | 150 kDa |
纯化方法 | Protein A purified |
物理外观 | Liquid |
储存缓冲液 | 59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3 |
防腐剂 | 0.01% Sodium azide |
浓度 | 0.5 mg/mL |
储存温度 | -20°C储存,避免反复冻融 |
运输条件 | 超低温冰袋运输 |
稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Application | Notes |
---|---|
Flow | 1/800 - 1/2000 |
WB |
1/1,000-1/20,000
|
IP |
1/50
|
WB |
1/1000 - 1/2000
|
IF/ICC | 1/1000 |
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Recombinant PCNA Antibody (Ab120681) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 14.1 s
Recombinant PCNA Antibody (Ab120681) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant PCNA Antibody (Ab120681) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant PCNA Antibody (Ab120681) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant PCNA Antibody (Ab120681) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant PCNA Antibody (Ab120681) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant PCNA Antibody (Ab120681) - IP
PCNA was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant PCNA Antibody (Ab120681) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant PCNA Antibody (Ab120681) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant PCNA Antibody (Ab120681) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).