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Recombinant ATP1A1 Antibody

功能和特点
  • 规格或纯度: Recombinant (RR685); Rabbit anti Human ATP1A1 Antibody; WB, IHC, Flow, IP; Unconjugated
  • 简短描述: Rabbit recombinant (RR685) to ATP1A1
  • 反应种属: Chinese hamster ,Human,Mouse ,Rat 
  • 亚型: Rabbit IgG
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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab128631-50μl
50μL 现货 Stock Image
Ab128631-100μl
100μL 现货 Stock Image
Ab128631-1ml
1ml 期货 Stock Image

基本信息

产品名称 Recombinant ATP1A1 Antibody
别名 ATPase Na+/K+ transporting alpha antibody; adenosinetriphosphatase antibody; AT1A1_HUMAN antibody; ATP1A1 antibody; ATP1A4 antibody; ATP1AL2 antibody; ATP1B antibody; ATP1B1 antibody; ATPase Na+/K+ transporting alpha 1 polypeptide antibody; ATPase Na+/K+
英文别名 ATPase Na+/K+ transporting alpha antibody; adenosinetriphosphatase antibody; AT1A1_HUMAN antibody; ATP1A1 antibody; ATP1A4 antibody; ATP1AL2 antibody; ATP1B antibody; ATP1B1 antibody; ATPase Na+/K+ transporting alpha 1 polypeptide antibody; ATPase Na+/K+
规格或纯度 Recombinant (RR685); Rabbit anti Human ATP1A1 Antibody; WB, IHC, Flow, IP; Unconjugated
特异性 ATP1A1
反应种属 Chinese hamster ,Human,Mouse ,Rat 
预测反应种属 Tilapia
交叉反应 Cross-reactivity with other species has not been measured.
免疫原 A synthetic peptide corresponding to residues on the N-terminus of human NaK ATPase was used as an immunogen.
阳性对照 WB: C6, HT29, A431, Hep G2, and Raw264.7 cell lysates. IHC: Endometrium tissue. Flow: HeLa cells. IP: TT whole cell lysate.
偶联标记 Unconjugated

产品属性

克隆 Recombinant
克隆号 RR685
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂 0.01% Sodium azide
浓度 0.5 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application Notes
WB

1/2000 - 1/5000

IP

1/50

Flow

1/50 - 1/200

IHC

1/10000 - 1/20000

质检证书(COA)

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图片

Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 32.2 s

Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 10.0 s

Recombinant ATP1A1 Antibody (Ab128631) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with Recombinant ATP1A1 Antibody (Ab128631) at 1/10000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant ATP1A1 Antibody (Ab128631) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ATP1A1 Antibody (Ab128631) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ATP1A1 Antibody (Ab128631) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

Recombinant ATP1A1 Antibody (Ab128631) - IP
NaK ATPase was immunoprecipitated from 0.4 mg of TT whole cell lysate with Recombinant ATP1A1 Antibody (Ab128631) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant ATP1A1 Antibody (Ab128631) IP in TT whole cell lysate
Lane 2: PBS instead of Recombinant ATP1A1 Antibody (Ab128631) in TT whole cell lysate
Lane 3: TT whole cell lysate, 10 μg (input)

Exposure: 120 s.

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