计算溶液所需的质量、体积或浓度。
这是演示店铺,请务下单付款,避免造成你的财物损失。
为了获得访问"阿拉丁铁蛋"实时聊天框的流畅支持体验,建议您使用Chrome浏览器或选择360浏览器极速模式(如何切换极速模式?),感谢您选择我们!
货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab128631-50μl |
50μL |
现货 ![]() |
| |
Ab128631-100μl |
100μL |
现货 ![]() |
| |
Ab128631-1ml |
1ml |
期货 ![]() |
|
产品名称 | Recombinant ATP1A1 Antibody |
---|---|
别名 | ATPase Na+/K+ transporting alpha antibody; adenosinetriphosphatase antibody; AT1A1_HUMAN antibody; ATP1A1 antibody; ATP1A4 antibody; ATP1AL2 antibody; ATP1B antibody; ATP1B1 antibody; ATPase Na+/K+ transporting alpha 1 polypeptide antibody; ATPase Na+/K+ |
英文别名 | ATPase Na+/K+ transporting alpha antibody; adenosinetriphosphatase antibody; AT1A1_HUMAN antibody; ATP1A1 antibody; ATP1A4 antibody; ATP1AL2 antibody; ATP1B antibody; ATP1B1 antibody; ATPase Na+/K+ transporting alpha 1 polypeptide antibody; ATPase Na+/K+ |
规格或纯度 | Recombinant (RR685); Rabbit anti Human ATP1A1 Antibody; WB, IHC, Flow, IP; Unconjugated |
特异性 | ATP1A1 |
反应种属 | Chinese hamster ,Human,Mouse ,Rat |
预测反应种属 | Tilapia |
交叉反应 | Cross-reactivity with other species has not been measured. |
免疫原 | A synthetic peptide corresponding to residues on the N-terminus of human NaK ATPase was used as an immunogen. |
阳性对照 | WB: C6, HT29, A431, Hep G2, and Raw264.7 cell lysates. IHC: Endometrium tissue. Flow: HeLa cells. IP: TT whole cell lysate. |
偶联标记 | Unconjugated |
克隆 | Recombinant |
---|---|
克隆号 | RR685 |
Format | Whole IgG |
亚型 | Rabbit IgG |
SDS-PAGE | 150 kDa |
纯化方法 | Protein A purified |
物理外观 | Liquid |
储存缓冲液 | 59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3 |
防腐剂 | 0.01% Sodium azide |
浓度 | 0.5 mg/mL |
储存温度 | -20°C储存,避免反复冻融 |
运输条件 | 超低温冰袋运输 |
稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Application | Notes |
---|---|
WB |
1/2000 - 1/5000
|
IP |
1/50
|
Flow | 1/50 - 1/200 |
IHC | 1/10000 - 1/20000 |
输入批号以搜索COA:
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 32.2 s
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 10.0 s
Recombinant ATP1A1 Antibody (Ab128631) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with Recombinant ATP1A1 Antibody (Ab128631) at 1/10000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant ATP1A1 Antibody (Ab128631) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ATP1A1 Antibody (Ab128631) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ATP1A1 Antibody (Ab128631) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant ATP1A1 Antibody (Ab128631) - IP
NaK ATPase was immunoprecipitated from 0.4 mg of TT whole cell lysate with Recombinant ATP1A1 Antibody (Ab128631) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant ATP1A1 Antibody (Ab128631) IP in TT whole cell lysate
Lane 2: PBS instead of Recombinant ATP1A1 Antibody (Ab128631) in TT whole cell lysate
Lane 3: TT whole cell lysate, 10 μg (input)
Exposure: 120 s.