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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab138202-100μl |
100μL | 期货 | | |
Ab138202-1ml |
1ml | 期货 | |
产品名称 | Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) |
---|---|
别名 | Goat Anti Rabbit IgG H&L (Alexa Fluor® 405); Goat Anti-Rabbit IgG H&L-Alexa Fluor® 405; Goat Anti Rabbit IgG H&L-Alexa Fluor® 405; Goat Anti-Rabbit IgG H&L (Alexa Fluor 405); Goat Anti Rabbit IgG H&L (Alexa Fluor 405); Goat Anti-Rabbit IgG H&L-Alexa Fluo |
英文别名 | Goat Anti Rabbit IgG H&L (Alexa Fluor® 405); Goat Anti-Rabbit IgG H&L-Alexa Fluor® 405; Goat Anti Rabbit IgG H&L-Alexa Fluor® 405; Goat Anti-Rabbit IgG H&L (Alexa Fluor 405); Goat Anti Rabbit IgG H&L (Alexa Fluor 405); Goat Anti-Rabbit IgG H&L-Alexa Fluo |
规格或纯度 | pAb; Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405); ICC, IF, IHC, Flow, ELISA |
免疫原 | Rabbit IgG |
偶联标记 | Alexa Fluor® 405 |
克隆 | Polyclonal |
---|---|
Format | Whole IgG |
亚型 | IgG |
纯化方法 | Antigen Affinity Purified |
物理外观 | Liquid |
储存缓冲液 | Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% Glycerol, 0.05% Sodium Azide |
防腐剂 | 0.05% Sodium Azide |
浓度 | 1.0 mg/mL |
储存温度 | -20°C储存,避免反复冻融 |
运输条件 | 超低温冰袋运输 |
稳定性与储存 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Application | Notes |
---|---|
ELISA |
1/5000 - 1/20000
|
IF |
1/100-1/1000
|
FLow |
1/500-1/1000
|
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (Ab138202) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) 1/1000. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (Ab138202) was used as the secondary antibody at 1/500 dilution. Images were taken on the confocal laser scanning microscope.