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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab138205-100μl |
100μL |
期货 ![]() |
| |
Ab138205-1ml |
1ml |
期货 ![]() |
|
产品名称 | Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) |
---|---|
别名 | Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488); Goat Anti Rabbit IgG H&L (Alexa Fluor® 488); Goat Anti-Rabbit IgG H&L-Alexa Fluor® 488; Goat Anti Rabbit IgG H&L-Alexa Fluor® 488; Goat Anti-Rabbit IgG H&L (Alexa Fluor 488); Goat Anti Rabbit IgG H&L (Alexa Flu |
英文别名 | Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488); Goat Anti Rabbit IgG H&L (Alexa Fluor® 488); Goat Anti-Rabbit IgG H&L-Alexa Fluor® 488; Goat Anti Rabbit IgG H&L-Alexa Fluor® 488; Goat Anti-Rabbit IgG H&L (Alexa Fluor 488); Goat Anti Rabbit IgG H&L (Alexa Flu |
规格或纯度 | pAb; Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488); ICC, IF, IHC, Flow, ELISA |
免疫原 | Rabbit IgG |
偶联标记 | Alexa Fluor® 488. Ex: 495nm, Em: 519nm |
克隆 | Polyclonal |
---|---|
Format | Whole IgG |
亚型 | IgG |
纯化方法 | Antigen Affinity Purified |
物理外观 | Liquid |
储存缓冲液 | Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide |
防腐剂 | 0.02% Sodium Azide |
浓度 | 1 mg/mL |
储存温度 | -20°C储存 |
运输条件 | 超低温冰袋运输 |
稳定性与储存 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Application | Notes |
---|---|
ELISA |
1/5000 - 1/20000
|
IF |
1/100-1/1000
|
FLow |
1/500-1/1000
|
输入批号以搜索COA:
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Ab138205) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) 1/1000. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Ab138205) was used as the secondary antibody at 1/500 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.