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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
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Ab156253-100μg (试用装) 申请此免费试用装(?) 作为尊贵的客户,您每年可免费申请一次试用装产品,尽享探索与享受!
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100μg |
现货 ![]() |
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Ab156253-1mg |
1mg |
现货 ![]() |
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产品名称 | Goat Anti-Mouse IgG H&L (Biotin) |
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别名 | Goat Anti-Mouse IgG H&L Antibody (Biotin); Anti-Mouse IgG H&L Antibody (Biotin); Mouse IgG H&L Antibody (Biotin); Biotin-conjugated Goat Anti-Mouse IgG (H+L); Goat Anti Mouse IgG Antibody (Biotin); Anti-Mouse IgG Antibody (Biotin); Mouse IgG Antibody (Bi |
英文别名 | Goat Anti-Mouse IgG H&L Antibody (Biotin); Anti-Mouse IgG H&L Antibody (Biotin); Mouse IgG H&L Antibody (Biotin); Biotin-conjugated Goat Anti-Mouse IgG (H+L); Goat Anti Mouse IgG Antibody (Biotin); Anti-Mouse IgG Antibody (Biotin); Mouse IgG Antibody (Bio |
规格或纯度 | Secondary Antibody; Goat Anti-Mouse IgG H&L (Biotin); WB, ELISA, IF, Flow |
特异性 | Mouse IgG |
交叉反应 | No cross reactivity with rabbit IgG, and human IgG. |
免疫原 | Mouse IgG |
阳性对照 | ELISA: Mouse IgG |
偶联标记 | Biotin |
克隆 | Polyclonal |
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Format | Whole IgG |
纯化方法 | Antigen affinity purified |
物理外观 | Liquid |
储存缓冲液 | Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300. |
防腐剂 | 0.05% Proclin300 |
浓度 | 1.0 mg/mL |
储存温度 | 2-8°C储存,避光,避免反复冻融 |
运输条件 | 冰袋运输 |
稳定性与储存 | Store at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Avoid repeated freeze/thaw cycles. Protect from light. |
Application | Notes |
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WB | 1/10000-1/200000 |
IF | 1:1000 |
ELISA | 1/20000-1/400000 |
输入批号以搜索COA:
Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.