Goat Anti-Human IgG (Cy5)

功能和特点

规格或纯度: Secondary Antibody; Goat Anti-Human IgG (Cy5); IF, Flow
简短描述: Secondary antibody, Goat Anti-Human IgG (Cy5)

有货

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
Ab175833-100μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	100μg	现货
Ab175833-1mg	1mg	现货

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基本信息

产品名称	Goat Anti-Human IgG (Cy5)
别名	Goat Anti-Human Antibody (Cy5); Cy5-conjugated Goat Anti-Human Antibody; Goat Anti-Human IgG / Cy5 antibody; Goat Anti-Human IgG-Cy5 antibody; Cy5-山羊抗人IgG; 山羊抗人IgG (Cy5);花菁染料Cy5标记的山羊抗人IgG 抗体
英文别名	Goat Anti-Human Antibody (Cy5); Cy5-conjugated Goat Anti-Human Antibody; Goat Anti-Human IgG / Cy5 antibody; Goat Anti-Human IgG-Cy5 antibody;
规格或纯度	Secondary Antibody; Goat Anti-Human IgG (Cy5); IF, Flow
特异性	Human IgG
偶联标记	105

产品属性

克隆	Polyclonal
Format	Whole IgG
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

应用

Application	Notes
Flow	1/100-1/1000
IF	1:1000

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图片

Flow Cytometry: Goat Anti-Human IgG (Cy5) (Ab175833)

Goat Anti-Human IgG (Cy5) (Ab175833) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Rituximab (anti-CD20) (R412001). Goat Anti-Human IgG (Cy5) (Ab175833) at a dilution of 1/100 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

IF: Goat Anti-Human IgG (Cy5) (Ab175833)

Goat Anti-Human IgG (Cy5) (Ab175833) - IF
IF analysis of EGFR (red) in A549 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Necitumumab (anti-EGFR) (Ab169264) at 2 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (Cy5) (Ab175833) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.