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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab179003-100μg |
100μg | 期货 | | |
Ab179003-1mg |
1mg | 期货 | |
产品名称 | Goat Anti-Mouse IgG H&L (FITC) |
---|---|
别名 | Goat Anti-Mouse IgG H&L Antibody (FITC); Anti-Mouse IgG H&L Antibody (FITC); Mouse IgG H&L Antibody (FITC); FITC-conjugated Goat Anti-Mouse IgG (H+L); Goat Anti Mouse IgG Antibody (FITC); Anti-Mouse IgG Antibody (FITC); Mouse IgG Antibody (FITC); Goat ant |
英文别名 | Goat Anti-Mouse IgG H&L Antibody (FITC); Anti-Mouse IgG H&L Antibody (FITC); Mouse IgG H&L Antibody (FITC); FITC-conjugated Goat Anti-Mouse IgG (H+L); Goat Anti Mouse IgG Antibody (FITC); Anti-Mouse IgG Antibody (FITC); Mouse IgG Antibody (FITC); Goat ant |
规格或纯度 | Secondary Antibody; Goat Anti-Mouse IgG H&L (FITC); IF, Flow |
特异性 | Mouse IgG |
交叉反应 | Reacting with mouse whole IgG (mouse IgG light chain, heavy chain), may cross react with immunoglobulins from other species. |
免疫原 | Mouse IgG |
偶联标记 | FITC |
克隆 | Polyclonal |
---|---|
Format | Whole IgG |
亚型 | Goat IgG |
SDS-PAGE | 150 kDa |
纯化方法 | Antigen affinity purified |
物理外观 | Liquid |
储存缓冲液 | Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300. |
防腐剂 | 0.05% Proclin300 |
浓度 | 1.0 mg/mL |
储存温度 | 避光,-20°C储存,避免反复冻融 |
运输条件 | 超低温冰袋运输 |
稳定性与储存 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
Application | Notes |
---|---|
IF | 1:1000 |
Flow |
1:100-1:2000
|
输入批号以搜索COA:
Goat Anti-Mouse IgG H&L (FITC) (Ab179003) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/1600 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Mouse IgG H&L (FITC) (Ab179003) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.