这是演示店铺，请务下单付款，避免造成你的财物损失。

为了获得访问"阿拉丁铁蛋"实时聊天框的流畅支持体验，建议您使用Chrome浏览器或选择360浏览器极速模式(如何切换极速模式?)，感谢您选择我们！

首页   400-620-6333
提交您使用阿拉丁产品的研究文章，就得100元奖励！立即访问我们的提交页面.
  • 英文站
  • 登录
切换导航
我的购物车
搜索
搜索444
高级搜索
菜单
登录
྾

Beta Galactosidase

功能和特点
  • 规格或纯度: Specific Activity >6 U/mg;Activity >3 U/ml
  • 生物活性: >3 U/ml
有货

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
B489898-20μl
 20μl 期货 Stock Image
B489898-60μl
 60μl 期货 Stock Image
大包装询价 添加到收藏夹 比较  SDS中文版  DATASHEET
main product photo

基本描述

产品名称 Beta Galactosidase
规格或纯度 Specific Activity >6 U/mg;Activity >3 U/ml
产品介绍


Product Description

Beta Galactosidase from Streptococcus pneumoniae releases only β(1-4)- linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. β(1-4) galactose is by far the most common linkage found in N-linked oligosaccharides. For other galactosidase linkages, ß(1-3,4,6)-Galactosidase from Bovine testes is recommended. The enzyme is as active on tetraantennary oligosaccharides as on disaccharides containing β(1-4)-linked galactose. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose. Up to 100 υg of asialofetuin can be completely β(1-4)-degalactosylated in less than 1 hour using 3 mU of enzyme.

Contents
ß-(1-4) Galactosidase in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).

Included with 20 µL and 60 µL pack sizes:
5x Reaction Buffer 6.0 (250 mM sodium phosphate, pH 6.0).

Molecular Weight

~250,000 daltons

pH optimum

6.0, active over the range 5-7.

The supplied buffer concentrate provides the optimal pH for enzyme activity with the standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity.

Formulation

The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).

Specific Activity

One unit of ß-(1-4)-galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C pH 5 from p-nitrophenyl-beta-D-galactopyranoside.

Specificity

Non-reducing terminal ß(1-4)-Galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

Stability

Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.

Purity

ß(1-4)-Galactosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.

The production host strain has been extensively tested and does not produce any detectable glycosidases.

Directions for use

1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.

2. Add deionized water to a total of 14 µl.

3. Add 4 µl of 5x Reaction Buffer 6.0.

4. Add 2 µl ß(1-4) Galactosidase.

5. Incubate at 37°C for 1 hour.

For glycoproteins, cleavage may be monitored by SDS-PAGE if the size differential between native and de-galactosylated protein is sufficient for detection.

Note: The optimum pH for cleavage of oligosaccharides is ~6.
生物活性 >3 U/ml
来源 Recombinant from Streptococcus pnuemonia in E.coli

产品规格参数

储存温度 2-8°C储存
运输条件 冰袋运输
酶学委员会编号 3.2.1.23

质检证书(COA)

质检报告(COA)

输入批号以搜索COA:

产品问答

产品问答

登录提交问题 Hover me 请先登录再提交问题
查看全部
您提交该产品问题后,我们会在1-2个工作日内给您答复,您可以登录"我的账号",然后点击"我的产品问答"查看答案

计算器

上海阿拉丁生化科技股份有限公司
销售条款及条件         隐私政策
© 2016 ALADDIN-E.COM . 版权所有,未经授权禁止拷贝本站资料,如有违反,将追究法律责任
沪公网安备 31012002004058号 沪ICP备09093490号-2

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。

上海工商 危险品化学品经营许可证（带存储）营业执照(三证合一)