这是演示店铺，请务下单付款，避免造成你的财物损失。

为了获得访问"阿拉丁铁蛋"实时聊天框的流畅支持体验，建议您使用Chrome浏览器或选择360浏览器极速模式(如何切换极速模式?)，感谢您选择我们！

首页   400-620-6333
提交您使用阿拉丁产品的研究文章，就得100元奖励！立即访问我们的提交页面.
  • 英文站
  • 登录
切换导航
我的购物车
搜索
搜索444
高级搜索
菜单
登录
྾

Endo-Beta-Galactosidase

规格或纯度: Specific Activity >140 U/mg;Activity >14 U/ml
有货

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
E489844-20μl
 20μl 期货 Stock Image
E489844-60μl
 60μl 期货 Stock Image
大包装询价 添加到收藏夹 比较  SDS中文版  DATASHEET
main product photo

基本描述

规格或纯度 Specific Activity >140 U/mg;Activity >14 U/ml
英文名称 Endo-Beta-Galactosidase
来源 recombinant gene from Bacteroides fragilis in E.Coli
储存温度 2-8°C储存
运输条件 冰袋运输
产品介绍


Product Description

Endo-Beta-Galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures. Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may decrease or eliminate cleavage.

Endo-Beta-Galactosidase is useful for identifying and removing poly-N-acetyllactosamine structures on many biologically important glycoconjugates.


Contents
60 µl aliquot of enzyme (0.9 U) in 20 mM tris-HCl, pH 7.5
1 vial reaction buffer- 250mM Sodium phosphate, pH 5.8

pH Optimum: 5.8

Molecular Weight

32,000 daltons

Formulation

The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, pH 7.5

Stability

Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity. Active at least 5 days under reaction conditions.

Specificity

Internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine [GlcNAcβ(1-3) Galβ(1-4)]n structures are the preferred substrate. Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may decrease or eliminate cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharidesof the neo-lacto group are cleaved at greatly educed rates depending on the deviation from the preferred substrate.

For example, Galβ(1-3)GlcNAcβ(1-3)Galβ(1-4)Glc is cleaved at 5x10-5 the rate of keratan sulfate(see ref.4). Specificity is similar to the Escherichia freundii enzyme. except that it is limited to cleaving N-acetyllactosamine extensions on tetraantennary structures of erythropoietin(see ref 5).

Specific Activity

One unit of endo-β-Galactosidase is defined as the amount that will liberate one μmole of reducing sugar per minute at 37℃and pH 5.8 from bovine corneal keratan sulfate. Purity Endo-β-Galactosidase is tested for contaminating protease as follows: 10 ug of denatured BSA is incubated for 24 hours at 37℃ with 2 μl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production strain of E. coli has been extensively tested and does not produce any detectable glycosidases.

Directions for use

For glycoproteins:

1. Add up to 100 μg of glycoprotein to a tube.

2. Add 4 ul 5X buffer and water to 19 μl.

3. Add 1 μl enzyme.

4. Incubate at 37℃ for 2 hrs.

Procedure for oligosaccharides:

Same as above except incubate from several hours to several days depending on the substrate. Add bovine serum albumen to 2 mg/ml to stabilize the protein during extended incubations.

Applications

Endo-β-Galactosidase (EC 3.2.1.103) cleaves internal β(1-4) galactose linkages in unbranched, repeating polyN-acetyllactosamine structures. Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may decrease or eliminate cleavage. Endo-β-Galactosidase is useful for identifying and removing poly-N-acetyllactosamine structures on many biologically important glycoconjugates. 

名称和标识符

酶学委员会编号 3.2.1.103

质检证书(COA)

质检报告(COA)

输入批号以搜索COA:

产品问答

产品问答

登录提交问题 Hover me 请先登录再提交问题
查看全部
您提交该产品问题后,我们会在1-2个工作日内给您答复,您可以登录"我的账号",然后点击"我的产品问答"查看答案

计算器

上海阿拉丁生化科技股份有限公司
销售条款及条件         隐私政策
© 2016 ALADDIN-E.COM . 版权所有,未经授权禁止拷贝本站资料,如有违反,将追究法律责任
沪公网安备 31012002004058号 沪ICP备09093490号-2

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。

上海工商 危险品化学品经营许可证（带存储）营业执照(三证合一)