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荧光素酶 来源于萤火虫

规格或纯度: Recombinant,≥2.0×10^11 RLU/mg protein,Lyophilized powder approximately 20% protein
有货

库存信息

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库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
L465180-1mg
1mg 现货 Stock Image
L465180-5mg
5mg 期货 Stock Image

基本描述

英文别名 Photinus-luciferin 4-monooxygenase (ATP-hydrolysing); firefly luciferase; luciferase (firefly luciferin); Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing); firefly luciferin luciferase; Photinus pyralis luciferase
规格或纯度 Recombinant,≥2.0×10^11 RLU/mg protein,Lyophilized powder approximately 20% protein
英文名称 Luciferase from Photinus pyralis (firefly)
单位定义 One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.
来源 大肠杆菌
储存温度 -80℃储存
运输条件 超低温冰袋运输
备注 Packaging 1mg refers to the quality of the protein.
产品介绍

分子量:mol wt 120 kDa (two subunits)

Product introduction

Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.


Application

The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.

名称和标识符

酶学委员会编号 1.13.12.7

安全和危险性(GHS)

象形图
ghs08

Health Hazard

ghs07

Harmful

信号词 Warning
危险声明 H373: Causes damage to organs through prolonged or repeated exposure
H302: Harmful if swallowed
预防措施声明 P264,P260,P270

质检证书(COA)

质检报告(COA)

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