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Phaseolus vulgaris lectin P (PHA-P), E-subunit enhanced

有货

库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
P419324-5mg
5mg 现货 Stock Image
P419324-10mg
10mg 现货 Stock Image

基本描述

英文名称 Phaseolus vulgaris lectin P (PHA-P), E-subunit enhanced
储存温度 -20°C储存
运输条件 超低温冰袋运输
产品介绍


Features 

Ultrapure quality

Agglutinates leucocytes and erythrocytes

Mixture of L and E subunits

Sugar specificity: complex oligosaccharides

Lyophilized powder

Product description

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containeningL (leucoagglutinin) and E (erythroagglutinin) subunits incompositions L1E3, L2E2, L3E1, E4.

Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides . PHA-P is not blood group specific and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.

Medicago’s PHA-P lectin is supplied as a white to lightpinkessentially salt-free lyophilized powder from 50 mMNH4HCO3, which evaporates during the lyophilizationprocedure. The purity of the lectin is determined by SDSPAGEgenerating one single band. 

Applications

Lateral flow erythrocyte separation

Typical subunit composition

Total L subunit composition: 35-40%

Total E subunit composition: 60-65%

L4 0-10%

L3E1 10-20%

L2E2 15-25%

L1E3 35-50%

E4 10-20%

Directions for use

The lectin may be reconstituted in a suitable buffer for yourapplication. 

Shipping and storage

The product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months.


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