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Phaseolus vulgaris lectin P (PHA-P), native isomer composition

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
P419332-5mg
5mg 现货 Stock Image
P419332-10mg
10mg 现货 Stock Image

基本描述

英文名称 Phaseolus vulgaris lectin P (PHA-P), native isomer composition
储存温度 -20°C储存
运输条件 超低温冰袋运输
产品介绍


Features 

Ultrapure quality

Agglutinates leucocytes and erythrocytes

Mixture of L and E subunits

Sugar specificity: complex oligosaccharides

Lyophilized powder

 

Product description

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containingL (leucoagglutinin) and E (erythroagglutinin) subunits incompositions L1E3, L2E2, L3E1, E4.

Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides . PHA-P is not blood group specific and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.

PHA-P lectin is supplied as a white to light-pinkessentially salt-free lyophilized powder from 50 mM NH4HCO3,which evaporates during the lyophilization procedure. Thepurity of the lectin is determined by SDS-PAGE generatingone single band.


Applications

Lateral flow erythrocyte separation

Typical subunit composition

Total L subunit composition: 60-65%

Total E subunit composition: 35-40%

L4 25-35%

L3E1 20-30%

L2E2 10-20%

L1E3 17-27%

E4 1-11%


Directions for use

The lectin may be reconstituted in a suitable buffer for yourapplication.


Shipping and storage

The product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months.


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