Recombinant Human CCL4L1 Protein

功能和特点

规格或纯度: Animal Free, >97%(SDS-PAGE, HPLC), Active, E.coli, No tag, 24-92 aa
生物活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
蛋白标签: No tag
应用: Cell Culture,Mass Spectrometry,Active,SDS-PAGE

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货号 (SKU)	包装规格	是否现货
rp143752-10μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	10μg	现货
rp143752-50μg	50μg	现货
rp143752-100μg	100μg	现货
rp143752-1mg	1mg	期货

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基本描述

产品名称	Recombinant Human CCL4L1 Protein
别名	重组人CCL4L1蛋白; AT744.2; C-C motif chemokine 4-like; CCL4L1; CCL4Lsmall inducible cytokine A4-like; chemokine (C-C motif) ligand 4-like 1; LAG1; LAG-1; LAG1CCL4L2; LAG-1chemokine (C-C motif) ligand 4-like; Lymphocyte activation gene 1 protein; Macrophage inf
英文别名	AT744.2; C-C motif chemokine 4-like; CCL4L1; CCL4Lsmall inducible cytokine A4-like; chemokine (C-C motif) ligand 4-like 1; LAG1; LAG-1; LAG1CCL4L2; LAG-1chemokine (C-C motif) ligand 4-like; Lymphocyte activation gene 1 protein; Macrophage inflammatory pro
规格或纯度	Animal Free, >97%(SDS-PAGE, HPLC), Active, E.coli, No tag, 24-92 aa
产品介绍	Purity: >97%(SDS-PAGE, HPLC) Function： Chemokine that induces chemotaxis of cells expressing CCR5 or CCR1. Inhibits HIV replication in peripheral blood monocytes that express CCR5. Background: CCL4, also known as MIP-1 beta, is a 12 kDa beta chemokine that is secreted by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. CCL4 interacts with CCR5 to attract lymphocytes, NK cells, and immature dendritic cells to sites of inflammation. CCL4 also blocks the entry of HIV into CCR5-expressing cells. Human CCL4 and its variants are encoded by two paralogous genes, one of which (SCYA4) encodes CCL4 itself. The other gene (SCYA4L) is found in two alleles that give rise to CCL4L1 and CCL4L2, respectively. The human CCL4L1 cDNA encodes a 92 amino acid (aa) precursor with a 23 aa signal sequence. Alternate splicing yields an isoform with a 40 aa internal deletion. The second allele (SCYA4L2), encoding CCL4L2, is spliced into a variety of isoforms. Human CC4L1 shares greater than 98% aa sequence identity with CCL4 and CCL4L2. It shares 96% aa sequence identity with rhesus CCL4 and approximately 80 - 90% aa sequence identity with bovine, mouse, rabbit, and rat CCL4. The gene copy number for CCL4L1 varies from zero to five, but this is not associated with changes in mRNA or protein levels. CCL4 and CCL4L1 demonstrate comparable CCR5 binding, promotion of chemotaxis, and inhibition of HIV replication in stimulated PBMC. In vivo, the first two N-terminal amino acids of CCL4 are removed by DPPIV. The truncated form is as active as full-length CCL4 and gains the ability to bind CCR1 and CCR2b. Similar proteolytic processing of CCL4L1 has not been described.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
纯度	＞97%(SDS-PAGE, HPLC)
表达系统	E.coli
种属	Human
氨基酸	24-92 aa
序列	APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
蛋白标签	No tag
蛋白长度	Full length protein
无动物源	Yes
Accession #	Q8NHW4
来源	Recombinant
预测分子量	7.8 kDa
SDS-PAGE	7.8 kDa

产品规格参数

应用	Cell Culture,Mass Spectrometry,Active,SDS-PAGE
物理外观	Lyophilized
储存缓冲液	Lyophilized from a 0.2 μm filtered concentrated solution in PBS.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 1.0 mg/mL. Stock solutions should be apportioned
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Protein Bioactivity: Recombinant Human CCL4L1 Protein (rp143752)

Recombinant Human CCL4L1 Protein (rp143752)-Protein Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/mL, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

SDS-PAGE: Recombinant Human CCL4L1 Protein (rp143752)

Recombinant Human CCL4L1 Protein (rp143752)-SDS-PAGE
Recombinant Human CCL4L1 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 7.8 kDa.

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Recombinant Human CCL4L1 Protein

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基本描述

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