Recombinant Human IL-12 Protein

功能和特点

规格或纯度: Animal Free, > 95% (SDS-PAGE), Active, CHO, No tag, Human IL-12 p40 (Ile23-Ser328) & Human IL-12 p35 (Arg23-Ser219)
蛋白标签: No tag

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货号 (SKU)	包装规格	是否现货
rp147384-20µg	20μg	现货
rp147384-50µg	50μg	现货
rp147384-100μg	100μg	期货
rp147384-1mg	1mg	期货

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基本描述

产品名称	Recombinant Human IL-12 Protein
别名	重组人白细胞介素-12蛋白; CLMF；CLMF p35；CLMF p40；CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lympho
英文别名	CLMF；CLMF p35；CLMF p40；CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration
规格或纯度	Animal Free, > 95% (SDS-PAGE), Active, CHO, No tag, Human IL-12 p40 (Ile23-Ser328) & Human IL-12 p35 (Arg23-Ser219)
产品介绍	白细胞介素-12 (IL-12)是一个70 kDa(p70蛋白)的异源二聚体细胞因子，由p35 (35 kDa) α 链和p40 (40 kDa) β 链两个共价连接的亚基组成。相对于p35链，p40链被过度生产，并形成同二聚体，与IL-12受体，与具有生物活性的p70异质二聚体竞争受体占用，从而作为受体拮抗剂。IL-12主要由活化的树突状细胞(DCs)、巨噬细胞和中性粒细胞在T细胞启动过程中产生，并直接作用于其细胞毒性免疫效应细胞，包括自然杀伤(NK)细胞、自然杀伤T (NKT)细胞及CD8+ T细胞，以刺激其增殖及增加其细胞毒性功能。IL-12被广泛认为是Th1反应的重要调节因子。它还促进激活的t细胞和NK细胞的扩增和存活，并调节ctl和NK细胞的细胞毒性活性。在适应性免疫应答过程中，IL-12启动抗原特异性t细胞以产生大量IFN-g(促使它们向Th-1通路)。IL-12还可以作为体液免疫的佐剂，通过增强IgG2a和IgG2b抗体的产生。重组人IL-12是一种69-75kDa的异二聚体糖蛋白(共503个氨基酸残基)，由35 kDa (p35)和40 kDa组成(p40)由二硫键连接的亚基。 Purity >95% SDS-PAGE. Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Background Interleukin-12 (IL-12) is a heterodimeric cytokine of 70 kDa(p70 protein) comprising two covalently linked subunits p35 (35 kDa) alpha (α) chain and the p40 (40 kDa) beta (β) chain. The p40 chain is overproduced relative to the p35 chain and forms homodimers that bind to the IL-12 receptor, competing with the bioactive p70 heterodimer for receptor occupancy and thereby serving as a receptor antagonist. IL-12 is predominantly produced by activated dendritic cells (DCs), macrophages, and neutrophils during T-cell priming and acts directly on cytotoxic immune effector cells, including natural killer (NK) cells, natural killer T (NKT) cells, and CD8+ T cells, to stimulate their proliferation and increase their cytotoxic functions. IL-12 is widely accepted as an important regulator of Th1 responses. It also promotes the expansion and survival of activated T-cells and NK cells and modulates the cytotoxic activity of CTLs and NK cells. During the adaptive immune response, IL-12 primes antigen-specific T-cells for high IFN-g production (driving their differentiation toward the Th-1 pathway). IL-12 can also act as an adjuvant for humoral immunity by enhancing production of IgG2a and IgG2b antibodies. Recombinant human IL-12 is a 69-75kDa isodimer glycoprotein (total of 503 amino acid residues) composed of 35 kDa (p35) and 40 kDa (p40) subunits linked by disulfide bonds.
纯度	＞ 95% (SDS-PAGE)
内毒素水平	< 0.01 EU/µg
表达系统	CHO cell
种属	Human
氨基酸	Human IL-12 p40 (Ile23-Ser328) +Linker(GSGSSRGGSGSGGSGGGGSK)+ Human IL-12 p35 (Arg23-Ser219)
序列	IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQV
蛋白标签	No tag
N-末端序列	Ile23
无动物源	Yes
Accession #	P29460 (IL-12p40), P29459 (IL-12p35)
预测分子量	59 kDa (p40-Linker-p35)
SDS-PAGE	69.8 kDa, under reducing conditions; 61.8 kDa, under non-reducing conditions.

产品规格参数

物理外观	Lyophilized
储存缓冲液	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute to a concentration of 0.1-1.0 mg/mL in sterile distilled H2O. Stock solutions should be apportioned into working aliquots and stored at-2
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Upon delivery aliquot, Store at -20°C, Avoid freeze / thaw cycles. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution.

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Protein Bioactivity: Recombinant Human IL-12 Protein (rp147384)

Recombinant Human IL-12 Protein (rp147384) - Protein Bioactivity
Recombinant human IL-12 bioactivity is measured in a cell proliferation assay using PHA-P-activated human peripheral blood mononuclear cell (PBMC), the ED₅₀ for this effect is less than 0.04654 ng/mL.

SDS-PAGE: Recombinant Human IL-12 Protein (rp147384)

Recombinant Human IL-12 Protein (rp147384) - SDS-PAGE
2.5μg/lane of Recombinant Human IL-12 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 69.8 kDa under reducing conditions and 61.8 kDa under non-reducing conditions.

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Recombinant Human IL-12 Protein

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基本描述

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