Recombinant Human Leptin Protein

功能和特点

规格或纯度: Animal Free, >97%(SDS-PAGE and HPLC), Active, E.coli, No tag, 22-167 aa
生物活性: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
蛋白标签: No tag

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货号 (SKU)	包装规格	是否现货
rp148245-100μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	100μg	现货
rp148245-1mg	1mg	现货
rp148245-200μg	200μg	现货
rp148245-5mg	5mg	期货

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基本描述

产品名称	Recombinant Human Leptin Protein
别名	重组人Leptin蛋白; FLJ94114; LEP; Leptin (murine obesity homolog); Leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS;FLJ94114;LEP_HUMAN;LEPD;Leptin;Leptin Murine Obesity Homolog;Leptin Precursor Obesity
英文别名	FLJ94114; LEP; Leptin (murine obesity homolog); Leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS;FLJ94114;LEP_HUMAN;LEPD;Leptin;Leptin Murine Obesity Homolog;Leptin Precursor Obesity Factor;Obesit
规格或纯度	Animal Free, >97%(SDS-PAGE and HPLC), Active, E.coli, No tag, 22-167 aa
产品介绍	Purity >97% SDS-PAGE. Recombinant human Leptin was overexpressed as insoluble protein aggregate in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Function May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
纯度	＞97%(SDS-PAGE and HPLC)
内毒素水平	＜1 EU/μg
表达系统	E.coli
种属	Human
氨基酸	22-167 aa
序列	VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC
蛋白标签	No tag
蛋白长度	Protein Fragment
无动物源	Yes
Accession #	P41159
来源	Recombinant
预测分子量	16 kDa

产品规格参数

物理外观	Lyophilized
储存缓冲液	Lyophilized from a 0.2 μm filtered concentrated solution in 50 mM PB, pH 3.5, with 0.02 % Tween-20.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

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Protein Bioactivity: Recombinant Human Leptin Protein (rp148245)

Recombinant Human Leptin Protein (rp148245)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0×10⁵ IU/mg

SDS-PAGE: Recombinant Human Leptin Protein (rp148245)

Recombinant Human Leptin Protein (rp148245)-SDS-PAGE
Recombinant Human Leptin Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 16.0 kDa.

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Recombinant Human Leptin Protein

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基本描述

产品规格参数

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