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Recombinant Human Lipocalin-2/NGAL Protein

Recombinant Human Lipocalin-2/NGAL Protein

功能和特点

规格或纯度: Animal Free, >97%(SDS-PAGE), Active, HEK293, C-His tag, 21-198 aa
蛋白标签: His tag at the C-terminus

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货号 (SKU)	包装规格	是否现货
rp148318-10μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	10μg	现货
rp148318-50μg	50μg	现货
rp148318-100μg	100μg	现货
rp148318-1mg	1mg	期货

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基本描述

产品名称	Recombinant Human Lipocalin-2/NGAL Protein
别名	重组人Lipocalin-2 / NGAL蛋白; 重组人脂质运载蛋白2; 24p3; 25 kDa alpha 2 microglobulin related subunit of MMP9; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Alpha 2 microglobulin related protein; HGNC:6526; HNL; Lcn 2; Lcn2; Lipocalin-2; Migration stimulating
英文别名	24p3; 25 kDa alpha 2 microglobulin related subunit of MMP9; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Alpha 2 microglobulin related protein; HGNC:6526; HNL; Lcn 2; Lcn2; Lipocalin-2; Migration stimulating factor inhibitor; MSFI; Neutrophil ge
规格或纯度	Animal Free, >97%(SDS-PAGE), Active, HEK293, C-His tag, 21-198 aa
产品介绍	Purity >97% SDS-PAGE. Actual molecular weight 25.7 kDa Molecular weight informat ~ 25.7 kDa (monomer). Human lipocalin-2 (aa 1-198) is fused at the C-terminus to a polyhistidine tag. Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
内毒素水平	< 1.0 EU/μg
表达系统	HEK293 Cells
种属	Human
氨基酸	21-198 aa
序列	QDSTSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLAGNAILREDKDPQKMYATIYELKEDKSYNVTSVLFRKKKCDYWIRTFVPGCQPGEFTLGNIKSYPGLTSYLVRVVSTNYNQHAMVFFKKVSQNREYFKITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDGADDDDKAHHHHHHHHHH
蛋白标签	His tag at the C-terminus
无动物源	Yes
Accession #	P80188
预测分子量	22.7 kDa
SDS-PAGE	24.6 kDa, under reducing conditions.

产品规格参数

物理外观	Lyophilized
储存缓冲液	Lyophilized from sterile 1*PBS, pH7.4, 5% Trehalose, 5% Mannitol, 0.01%Tween-80.
复溶	It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Samples are stable for twelve months from date of receipt at -20℃ to -80℃.Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.

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Protein Bioactivity: Recombinant Human Lipocalin-2/NGAL Protein (rp148318)

Recombinant Human Lipocalin-2/NGAL Protein (rp148318) - Protein Bioactivity
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2/NGAL Protein (rp148318). Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3.

Iron III (Fe3+): Aladdin, Cat# I115411
2,3,Dihydroxybenzoic Acid (DHBA): Aladdin, Catalog # D154867

SDS-PAGE: Recombinant Human Lipocalin-2/NGAL Protein (rp148318)

Recombinant Human Lipocalin-2/NGAL Protein (rp148318) - SDS-PAGE
3 μg/lane of Recombinant Human Lipocalin-2/NGAL was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 24.6 kDa.

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Recombinant Human Lipocalin-2/NGAL Protein

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基本描述

产品规格参数

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