Recombinant Human TNF-alpha Protein

功能和特点

规格或纯度: GMP, ≥95% (SDS-PAGE), Active, E.coli, No tag, 77-233 aa
生物活性: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The ED₅₀ for this effect is typically <0.05 ng/mL.
蛋白标签: No tag

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货号 (SKU)	包装规格	是否现货
rp152506-10μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	10μg	现货
rp152506-50μg	50μg	现货
rp152506-100μg	100μg	现货
rp152506-1mg	1mg	期货

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基本描述

产品名称	Recombinant Human TNF-alpha Protein
别名	重组人tnfα蛋白; 重组人肿瘤坏死因子α蛋白（TNF-alpha),TNF α; APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumo
英文别名	TNF α; APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily,
规格或纯度	GMP, ≥95% (SDS-PAGE), Active, E.coli, No tag, 77-233 aa
产品介绍	肿瘤坏死因子α（TNF α）是一种主要由巨噬细胞和单核细胞产生的细胞因子，可参与机体的免疫反应和发炎反应。TNF α值的升高败血症、恶性肿瘤、心脏衰竭和慢性炎性疾病有着密切的关系，可作为病情、治疗效果以及预后的评估指标。同时， TNF α还是自身免疫系统疾病的关键靶点，已有多种针对TNF α开发的单抗药物上市; Purity ≥ 95% SDS-PAGE Additional sequence information Full length mature chain soluble form. N-terminal glycine. Function Tumor necrosis factor-α (TNFα) is a kind of cytokine mainly produced by macrophages and monocytes, which can participate in the immune response and inflammatory response of the body. The increase of TNFα value is closely related to sepsis, malignant tumor, heart failure and chronic inflammatory diseases, which can be used as an evaluation index of disease condition, treatment effect and prognosis. At the same time, TNFα is also a key target of autoimmune diseases. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Post-translational The soluble form derives from the membrane form by proteolytic processing. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
生物活性	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The ED₅₀ for this effect is typically <0.05 ng/mL.
表达系统	E.coli
种属	Human
氨基酸	77 to 233
序列	VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVV PSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSP CQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQV YFGIIAL
蛋白标签	No tag
N-末端序列	Val
蛋白长度	Full length protein
Accession #	P01375
来源	Recombinant
预测分子量	17.4 kD

产品规格参数

物理外观	液体
储存缓冲液	10mM PBS，10%甘油，pH=7.4
浓度	Lot by Lot
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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Protein Bioactivity: Recombinant Human TNF-alpha Protein (rp152506)

Recombinant Human TNF-alpha Protein (rp152506)-Protein Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The EC₅₀ for this effect is typically <0.05ng/mL.

SDS-PAGE: Recombinant Human TNF-alpha Protein (rp152506)

Recombinant Human TNF-alpha Protein (rp152506)-SDS-PAGE
2μg/lane of Recombinant Human TNF-alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a single band at 16 kDa.

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Recombinant Human TNF-alpha Protein

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基本描述

产品规格参数

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