Recombinant Human EGF Protein (Biotin)

功能和特点

规格或纯度: EGF-Biotin, Active, Pichia pastoris, No tag, 971-1021 aa
生物活性: Binding EGFR
蛋白标签: No tag
应用: Flow,IF

有货

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
rp170191-20μg	20μg	现货

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基本描述

产品名称	Recombinant Human EGF Protein (Biotin)
别名	重组人表皮生长因子蛋白(EGF)；beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone
英文别名	beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone
规格或纯度	EGF-Biotin, Active, Pichia pastoris, No tag, 971-1021 aa
产品介绍	Introduction: Epidermal growth factor (EGF) is a hormone that stimulates division of epidermal and other cells. Labeling with biotinylated EGF can be followed by second-step labeling with streptavidin conjugates. Biotinylated EGF has enabled researchers to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Conjugation: The EGF was conjugated with Biotin under optimum conditions, and unreacted Biotin was removed. Recommended Usage： Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.5µg per million cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for different cell lines.
生物活性	Binding EGFR
表达系统	Pichia pastoris
种属	Human
氨基酸	971 - 1021 aa
序列	NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWE
蛋白标签	No tag
蛋白长度	Full length protein
来源	Recombinant

产品规格参数

应用	Flow,IF
物理外观	Lyophilized
储存缓冲液	Lyophilized from PBS (pH 7.4) buffer containing 1% BSA
防腐剂	No
复溶	This product should be reconstituted in 100μL of deionized water to make a 0.2mg/mL stock solution. Prepare working dilution on day of use. Stock solution is stable for about 8 weeks at 2-8°C as an undiluted liquid. Avoid freeze-thaw cycles. Protect from
储存温度	避光,-20°C储存
运输条件	冰袋运输
稳定性与储存	The lyophilized products should be stored at –20°C until use. Allow the product to warm to room temperature before opening. Protect from light. When stored properly, the products should be stable for at least 12 months.

图片

Flow Cytometry: Recombinant Human EGF Protein (Biotin) (rp170191)

Recombinant Human EGF Protein (Biotin) (rp170191) - Flow Cytometry
Flow analysis of EGF receptors (red line) in A431 Cells. Cells were labeled with Recombinant Human EGF Protein (Biotin) (rp170191) at 1/2500 dilution for 1 hour at 4°C, and then incubated with Streptavidin protein (APC) (np169048) at a dilution of 1/400 for 30 minutes at 4°C in the dark. Unlabeled Cells (black line) were used as a negative control.

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Recombinant Human EGF Protein (Biotin)

库存信息

基本描述

产品规格参数

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